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ExoQuick exosome precipitation solution (5 ml)
ExoQuick is a trusted, efficient solution for isolating exosomes from biofluids such as plasma, serum, urine, and cell culture media. Designed for simplicity, it eliminates the need for ultracentrifugation, enabling fast and reliable exosome precipitation. The 5 ml format is ideal for small to mid-sized studies, providing high exosome yield and purity. This user-friendly reagent is compatible with downstream applications like protein analysis, RNA extraction, and functional studies, making it a versatile choice for researchers in exosome research.
Référence interne:
EXOQ5A-1
How to Use ExoQuick Exosome Precipitation Solution (5 ml)
ExoQuick simplifies exosome isolation with an easy-to-follow protocol. Here’s a step-by-step guide:
- Sample Preparation: Collect the desired biofluid (plasma, serum, urine, or cell culture media) and centrifuge it to remove debris.
- Mixing: Add the appropriate volume of ExoQuick solution to the biofluid (as specified in the protocol). Mix thoroughly by inverting the tube.
- Incubation: Incubate the mixture at 4°C for the recommended time, allowing exosomes to precipitate efficiently.
- Centrifugation: Centrifuge the sample to pellet the exosomes. Carefully discard the supernatant.
- Resuspension: Resuspend the exosome pellet in a buffer or solution suitable for downstream analysis, such as RNA extraction or protein analysis.
Tips:
- Ensure samples are properly centrifuged before adding ExoQuick to remove large debris.
- Follow the provided protocol for optimal results.